Composite
R&FHis-tag

Part:BBa_K4422017:Design

Designed by: Grecia García-Elizalde   Group: iGEM22_UAM   (2022-09-30)


Removable and flexible purification 8X His-tag (8XHis-tag/Linker/Xa Factor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Flexible polyhistidine tag, which is flexible preventing steric hindrance. In case the tag has an effect in the protein, it can be removed after purification. This is an ideal tag for peptides.


Source

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References