Composite
R&FHis-tag
Part:BBa_K4422017:Design
Designed by: Grecia García-Elizalde Group: iGEM22_UAM (2022-09-30)
Removable and flexible purification 8X His-tag (8XHis-tag/Linker/Xa Factor)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Flexible polyhistidine tag, which is flexible preventing steric hindrance. In case the tag has an effect in the protein, it can be removed after purification. This is an ideal tag for peptides.
Source
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